RT Journal Article
T1 Differences in platelet‐rich plasma composition influence bone healing
A1 Al‐Hamed, Faez Saleh
A1 Abu‐Nada, Lina
A1 Rodan, Rania
A1 Sarrigiannidis, Stylianos
A1 Ramirez‐Garcialuna, José Luis
A1 Moussa, Hanan
A1 Elkashty, Osama
A1 Gao, Qiman
A1 Basiri, Tayebeh
A1 Baca González, Laura
A1 Torres García Denche, Jesús
A1 Rancán, Lisa
A1 Tran, Simon D.
A1 Lordkipanidzé, Marie
A1 Kaartinen, Mari
A1 Badran, Zahi
A1 Tamimi, Faleh
AB Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP(L-PRP) composition on bone defect healing.Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology.Results: Double-spin L-PRP contained higher concentrations of platelets than singlespin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP.Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.
PB Wiley
SN 0303-6979
SN 1600-051X
YR 2021
FD 2021-09-27
LK https://hdl.handle.net/20.500.14352/93312
UL https://hdl.handle.net/20.500.14352/93312
LA eng
NO Al-Hamed, F. S., Abu-Nada, L., Rodan, R., Sarrigiannidis, S., Ramirez-Garcialuna, J. L., Moussa, H., Elkashty, O., Gao, Q., Basiri, T., Baca, L., Torres, J., Rancan, L., Tran, S. D., Lordkipanidzé, M., Kaartinen, M., Badran, Z., Tamimi, F. Differences in platelet-rich plasma composition influence bone healing. Journal of Clinical Periodontology. 2021;48:1613-1626
DS Docta Complutense
RD 28 sept 2024