RT Journal Article
T1 Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants
A1 Yang, Fen
A1 Fernández Jiménez, Nadia
A1 Tučková, Martina
A1 Vrána, Jan
A1 Cápal, Petr
A1 Díaz, Mariana
A1 Pradillo Orellana, Mónica
A1 Pecinka, Ales
AB Structural maintenance of chromosome 5/6 (SMC5/6) complex is a crucial factor for preserving genome stability. Here, we show that mutants for several Arabidopsis (Arabidopsis thaliana) SMC5/6 complex subunits produce triploid offspring. This phenotype is caused by a meiotic defect leading to the production of unreduced male gametes. The SMC5/6 complex mutants show an absence of chromosome segregation during the first and/or the second meiotic division, as well as a partially disorganized microtubule network. Importantly, although the SMC5/6 complex is partly required for the repair of SPO11-induced DNA double-strand breaks, the nonreduction described here is SPO11-independent. The measured high rate of ovule abortion suggests that, if produced, such defects are maternally lethal. Upon fertilization with an unreduced pollen, the unbalanced maternal and paternal genome dosage in the endosperm most likely causes seed abortion observed in several SMC5/6 complex mutants. In conclusion, we describe the function of the SMC5/6 complex in the maintenance of gametophytic ploidy in Arabidopsis.
PB Oxford University Press
SN 1040-4651
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/99029
UL https://hdl.handle.net/20.500.14352/99029
LA eng
NO Fen Yang, Nadia Fernández-Jiménez, Martina Tučková, Jan Vrána, Petr Cápal, Mariana Díaz, Mónica Pradillo, Ales Pecinka, Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants, The Plant Cell, Volume 33, Issue 9, September 2021, Pages 3104–3119, https://doi.org/10.1093/plcell/koab178
NO Czech Science Foundation
NO Ministry of Education Youth and Sports (Czech Republic)
NO Palacký University  (Czech Republic)
NO China Scholarship Council Fellowship
NO European Commission
NO Ministerio de Educación (España)
NO International Plant Nucleus Consortium
DS Docta Complutense
RD 11 abr 2025