RT Journal Article
T1 Spectrally matched upconverting luminescent nanoparticles for monitoring enzymatic reactions
A1 Wilhelm, Stefan
A1 Del Barrio Redondo, Melisa
A1 Heiland, Josef
A1 Himmelstoß, Sandy
A1 Galbán, Javier
A1 Wolfbeis, Otto
A1 Hirsch, Thomas
AB We report on upconverting luminescent nanoparticles (UCLNPs) that are spectrally tuned such that their emission matches the absorption bands of the two most important species associated with enzymatic redox reactions. The core-shell UCLNPs consist of a β-NaYF4core doped with Yb3+/Tm3+ions and a shell of pure β-NaYF4. Upon 980 nm excitation, they display emission bands peaking at 360 and 475 nm, which is a perfect match to the absorption bands of the enzyme cosubstrate NADH and the coenzyme FAD, respectively. By exploiting these spectral overlaps, we have designed fluorescent detection schemes for NADH and FAD that are based on the modulation of the emission intensities of UCLNPs by FAD and NADH via an inner filter effect.
PB American Chemical Society
SN 1944-8244
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/93066
UL https://hdl.handle.net/20.500.14352/93066
LA eng
NO Stefan Wilhelm, Melisa del Barrio, Josef Heiland, Sandy F. Himmelstoß, Javier Galbán, Otto S. Wolfbeis, and Thomas HirschACS Applied Materials & Interfaces 2014 6 (17), 15427-15433DOI: 10.1021/am5038643
NO Consejo Superior de Investigaciones Científicas
NO Ministerio de Economía y Competitividad (España)
NO German Research Foundation
DS Docta Complutense
RD 10 abr 2025