RT Journal Article
T1 CRISPR/Cas9-mediated genome editing assists protein dynamics studies in live cells.
A1 Carrasco-Padilla, Carlos
A1 Roda Navarro, Pedro
AB Spatial and temporal regulation of molecular reactions dictates cell fate. Thus, studying molecular dynamics is essential to understand how cells decide what to do and the fundamental perturbations causing disease. Classically, molecular dynamics has been studied by protocols based in the overexpression of fluorescent fusion proteins. However, overexpression is associated to altered stoichiometry, molecular dynamics and subcellular distribution. We here discuss the necessity to study molecular dynamics of fluorescent fusion proteins expressed under physiological mechanisms in the cell, pointing to CRISPR/Cas9-mediated genome editing as the ideal means to do so. Current genome editing protocols enable us to study molecular dynamics while avoiding drawbacks associated to overexpression.
PB Elsevier
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/120058
UL https://hdl.handle.net/20.500.14352/120058
LA eng
NO Carrasco-Padilla C, Roda-Navarro P. CRISPR/Cas9-mediated genome editing assists protein dynamics studies in live cells. European Journal of Cell Biology 2022;101:151203. https://doi.org/10.1016/j.ejcb.2022.151203
DS Docta Complutense
RD 15 dic 2025