RT Journal Article T1 Effects of Fasting on Intermediary Metabolism Enzymes in the Liver and Muscle of Rainbow Trout A1 Fernández-Muela Garrote, Montserrat A1 Bermejo Poza, Rubén A1 Cabezas Albéniz, Almudena A1 Pérez, Concepción A1 González De Chávarri Echániz, Elisabeth A1 Díaz, María Teresa A1 Torrent, Fernando A1 Villarroel, Morris A1 Fuente Vázquez, Jesús De La AB Fish can go through periods of feed deprivation, either due to natural causes influenced by temperature or management techniques in animal production, affecting their energy metabolism differently, depending on the duration of fasting in days and water temperature. This study showed the effect of different days of fasting before slaughter in rainbow trout by analyzing the intermediary metabolic enzymes in the muscle and liver. For this purpose, a non-fasting group was compared with trout that fasted for 5, 10, and 20 days (55, 107, and 200 degree days (°C d), respectively). A first phase of increased activity of enzymes involved in glycolysis was observed, increasing hexokinase enzyme activity in muscle and pyruvate kinase enzyme activity in muscle and liver. As the fasting days progressed, enzymes involved in the gluconeogenic and glycogenolytic pathways in the liver such as lactate dehydrogenase and glycogen phosphorylase increased their activity regarding the no fasted group. After 20 days of fasting (200 °C d), lipogenic and protein related enzyme activity depletion was observed, leading to significant changes in energy metabolism. Therefore, prolonged fasting with high degree days duration may compromise the energy supply for the vital development of rainbow trout. PB MPDI SN 2410-3888 YR 2023 FD 2023-01-14 LK https://hdl.handle.net/20.500.14352/73191 UL https://hdl.handle.net/20.500.14352/73191 LA eng NO MINECO DS Docta Complutense RD 10 abr 2025