RT Journal Article T1 TNAP and P2X7R: New Plasma Biomarkers for Alzheimer’s Disease A1 Aivar, Paloma A1 Bianchi, Carolina A1 Di Lauro, Caterina A1 Soria Tobar, Lucía A1 Álvarez Castelao, Beatriz A1 Calero, Miguel A1 Medina, Miguel A1 Díaz Hernández, Miguel AB Over the last few years, intense research efforts have been made to anticipate or improve the diagnosis of Alzheimer’s disease by detecting blood biomarkers. However, the most promising blood biomarkers identified to date have some limitations, most of them related to the techniques required for their detection. Hence, new blood biomarkers should be identified to improve the diagnosis of AD, better discriminate between AD and mild cognitive impairment (MCI) and identify cognitively unimpaired (CU) older individuals at risk for progression to AD. Our previous studies demonstrated that both the purinergic receptor P2X7 and the tissue-nonspecific alkaline phosphatase ectoenzyme (TNAP) are upregulated in the brains of AD patients. Since both proteins are also present in plasma, we investigated whether plasma P2X7R and TNAP are altered in MCI and AD patients and, if so, their potential role as AD biomarkers. We found that AD but not MCI patients present increased plasma P2X7R levels. Nevertheless, TNAP plasma activity was increased in MCI patients and decreased in the AD group. ROC curve analysis indicated that measuring both parameters has a reasonable discriminating capability to diagnose MCI and AD conditions. In addition to confirming that individuals progressing to MCI have increased TNAP activity in plasma, longitudinal studies also revealed that CU individuals have lower plasma TNAP activity than stable controls. Thus, we propose that P2X7 and TNAP could serve as new plasma biomarkers for MCI and AD. PB MDPI SN 1422-0067 YR 2023 FD 2023-06-30 LK https://hdl.handle.net/20.500.14352/104603 UL https://hdl.handle.net/20.500.14352/104603 LA eng NO Aivar, P.; Bianchi, C.; Di Lauro, C.; Soria-Tobar, L.; Alvarez-Castelao, B.; Calero, M.; Medina, M.; Diaz-Hernandez, M. TNAP and P2X7R: New Plasma Biomarkers for Alzheimer’s Disease. Int. J. Mol. Sci. 2023, 24, 10897. https://doi.org/10.3390/ijms241310897 NO 2023 Descuento MDPI NO Ministerio de Economía y Competitividad (España) NO Ministerio de Ciencia e Innovación (España) NO European Commission NO UCM - Banco de Santander NO Comunidad de Madrid DS Docta Complutense RD 10 abr 2025