RT Journal Article
T1 Innate Lymphoid cells groups 1 and 3 in the epitelial compartment of functional human intestinal allografts
T2 Innate Lymphoid cells groups 1 and 3 in the epitelial compartment of functional human intestinal allografts
A1 Talayero, Paloma
A1 Mancebo Sierra, María Esther
A1 Calvo Pulido, Jorge
A1 Rodríguez Muñoz, Sarbelio
A1 Bernardo, I.
A1 Laguna Goya, Rocio
A1 Cano Romero, Francisco
A1 García-Sesma Pérez-Fuentes, Álvaro
A1 Loinaz Segurola, Carmelo
A1 Jiménez Romero, Luis Carlos
A1 Justo Alonso, Iago
A1 Paz Artal, Estela Natividad
AB We examined intraepithelial lymphocytes (IELs) in 213 ileal biopsies from 16 bowel grafts and compared them with 32 biopsies from native intestines. During the first year posttransplantation, grafts exhibited low levels of IELs (percentage of CD103(+) cells) principally due to reduced CD3(+) CD8(+) cells, while CD103(+) CD3(-) cell numbers became significantly higher. Changes in IEL subsets did not correlate with histology results, isolated intestine, or multivisceral transplants, but CD3(-) IELs were significantly higher in patients receiving corticosteroids. Compared with controls, more CD3(-) IELs of the grafts expressed CD56, NKp44, interleukin (IL)-23 receptor, retinoid-related orphan receptor gamma t (RORγt), and CCR6. No difference was observed in granzyme B, and CD3(-) CD127(+) cells were more abundant in native intestines. Ex vivo, and after in vitro activation, CD3(-) IELs in grafts produced significantly more interferon (IFN)-γ and IL-22, and a double IFNγ(+) IL-22(+) population was observed. Epithelial cell-depleted grafts IELs were cytotoxic, whereas this was not observed in controls. In conclusion, different from native intestines, a CD3(-) IEL subset predominates in grafts, showing features of natural killer cells and intraepithelial ILC1 (CD56(+) , NKp44(+) , CCR6(+) , CD127(-) , cytotoxicity, and IFNγ secretion), ILC3 (CD56(+) , NKp44(+) , IL-23R(+) , CCR6(+) , RORγt(+) , and IL-22 secretion), and intermediate ILC1-ILC3 phenotypes (IFNγ(+) IL-22(+) ). Viability of intestinal grafts may depend on the balance among proinflammatory and homeostatic roles of ILC subsets.
PB Elsevier
SN 1600-6135
YR 2016
FD 2016-01-15
LK https://hdl.handle.net/20.500.14352/98794
UL https://hdl.handle.net/20.500.14352/98794
LA eng
NO Talayero P, Mancebo E, Calvo-Pulido J, Rodríguez-Muñoz S, Bernardo I, Laguna-Goya R, Cano-Romero FL, García-Sesma A, Loinaz C, Jiménez C, Justo I, Paz-Artal E. Innate Lymphoid Cells Groups 1 and 3 in the Epithelial Compartment of Functional Human Intestinal Allografts. Am J Transplant. 2016 Jan;16(1):72-82. doi: 10.1111/ajt.13435
NO Instituto de Salud Carlos III
NO Unión Europea
NO Fundación Mutua Madrileña
DS Docta Complutense
RD 15 abr 2025