RT Journal Article
T1 Magneto-controlled electrochemical immunosensing platform to assess the senescence-associated GDF-15 marker in colorectal cancer
A1 Tejerina Miranda, Sandra
A1 Pérez Ginés, Víctor
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Pedrero Muñoz, María
A1 Montero Calle, Ana
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
AB In this study, we report a novel electrochemical immunoplatform using magnetic micro-supports and screen-printed carbon electrodes (SPCEs), overcoming limitations of the methods reported to date, for the rapid and sensitive determination of GDF-15, a molecular marker associated with cellular senescence in aging and cancer development and prognosis. The immunoplatform incorporated a sandwich-type configuration with specific capture and biotinylated-detection antibodies and used a streptavidin–peroxidase (strep–HRP) enzymatic conjugate as label. After magnetic capturing the micro-supports with the sandwich HRP-labeled immunocomplexes onto the surface of a SPCE, the change in cathodic current was quantified in the presence of H2O2 and hydroquinone (HQ), showing a direct correlation with the GDF-15 concentration. The proposed bioplatform exhibited attractive performance characteristics, including a good reproducibility (RSD 4.3%), and a wide linear concentration dynamic range from 140 to 10 000 pg mL−1 with a low limit of detection (LOD) of 42 pg mL−1 for GDF-15 standards in buffered solutions. The selectivity of the developed method and the storage stability of the capturing immunoconjugates were noteworthy. Indeed, the immunoconjugates showed a reliable performance for over 28 days when stored in a refrigerator. The immunoplatform was applied to a cohort of 19 plasma samples representing the different stages of colorectal cancer (CRC). The method allowed efficient discrimination between healthy individuals and CRC patients, particularly those in advanced stages, within a rapid 75-minute timeframe. The immunoplatform also presents substantial advantages in terms of costeffectiveness, assay time reduction, and simplicity compared to other available techniques.
PB Royal Society of Chemistry
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/109098
UL https://hdl.handle.net/20.500.14352/109098
LA eng
NO S. Tejerina-Miranda, V. Pérez-Ginés, R. M. Torrente-Rodríguez, M. Pedrero, A. Montero-Calle, J. M. Pingarrón, R. Barderas and S. Campuzano, Magneto-controlled electrochemical immunosensing platform to assess the senescence-associated GDF-15 marker in colorectal cancer, Sens. Diagn., 2024, 3, 238–247.
NO 2024 Acuerdos transformativos CRUE
NO Federación Española de Enfermedades Raras
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 26 feb 2026