RT Journal Article
T1 An electrochemical immunosensor using gold nanoparticles-PAMAM-nanostructured screen-printed carbon electrodes for tau protein determination in plasma and brain tissues from Alzheimer patients
A1 Razzino, Claudia A.
A1 Serafín González-Carrato, Verónica
A1 Gamella Carballo, María
A1 Pedrero Muñoz, María
A1 Montero Calle, Ana
A1 Barderas Manchado, Rodrigo
A1 Yáñez-Sedeño Orive, Paloma
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB This work reports a new sensitive strategy for the determination of tau protein, a hallmark of Alzheimer's disease (AD), involving a sandwich immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs) modified with a gold nanoparticles-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM) covalently immobilized onto electrografted p-aminobenzoic acid (p-ABA). The capture antibody (CAb) was immobilized by crosslinking with glutaraldehyde (GA) on the amino groups of the 3D-Au-PAMAM-p-ABA-SPCE, where tau protein was sandwiched with a secondary antibody labeled with horseradish peroxidase (HRP-DAb). Amperometry at −200 mV (vs the Ag pseudo-reference electrode) upon the addition of hydroquinone (HQ) as electron transfer mediator and H2O2 as the enzyme substrate was used to detect the immunocomplex formation. The great analytical performance of the immunosensor in terms of selectivity and low limit of detection (LOD) (1.7 pg mL−1) allowed the direct determination of the target protein in raw plasma samples and in brain tissue extracts from healthy individuals and post mortem diagnosed AD patients, using a simple and fast protocol.
PB Elsevier
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/114290
UL https://hdl.handle.net/20.500.14352/114290
LA eng
NO Claudia A. Razzino, Verónica Serafín, Maria Gamella, María Pedrero, Ana Montero-Calle, Rodrigo Barderas, Miguel Calero, Anderson O. Lobo, Paloma Yáñez-Sedeño, Susana Campuzano, José M. Pingarrón, An electrochemical immunosensor using gold nanoparticles-PAMAM-nanostructured screen-printed carbon electrodes for tau protein determination in plasma and brain tissues from Alzheimer patients, Biosensors and Bioelectronics, Volume 163, 2020, 112238.
DS Docta Complutense
RD 23 abr 2025