RT Journal Article
T1 Optimization of a Microfluidic Mixer for StudyingProtein Folding Kinetics
A1 Hertzog, David E.
A1 Ivorra, Benjamín Pierre Paul
A1 Mohammadi, Bijan
A1 Bakajin, Olgica
A1 Santiago, Juan G.
AB We have applied an optimization method in conjunction with numerical simulations to minimize the mixing time of a microfluidic mixer developed for protein folding studies. The optimization method uses a semideterministic algorithm to find the global minimum of the mixing time by varying the mixer geometry and flow conditions. We describe the minimization problem and constraints and give a brief overview of the optimization algorithm. We present results of the optimization, including the optimized geometry and parameter sensitivities, and we demonstrate the improvement in mixing performance with experiments using microfabricated mixers. The dyequenchin experiments of the original and optimized mixer designs show respective mixing times of 7 and 4 µs, a 40% eduction. The new design also provides more uniform mixing across streamlines that enter the mixer. The optimized mixer is the fastest reported continuous flow mixer for protein folding.
PB American Chemical society
SN 1520-6882
YR 2006
FD 2006-07-01
LK https://hdl.handle.net/20.500.14352/51330
UL https://hdl.handle.net/20.500.14352/51330
LA eng
NO NHI
NO U.S. Department of Energy
NO National Science Foundation. Center for Biophotonics,
NO NSF Science and Technology Center
DS Docta Complutense
RD 10 abr 2025