RT Journal Article
T1 Proneurogenic actions of follicle-stimulating hormone on neurospheres derived from ovarian cortical cells in vitro
A1 González Gil, Alfredo
A1 Sánchez Maldonado, María Belén
A1 Rojo Salvador, Concepción
A1 Flor García, Miguel
A1 Queiroga, Felisbina Luisa
A1 Ovalle, Susana
A1 Ramos Ruiz, Ricardo
A1 Fuertes Recuero, Manuel
A1 Picazo González, Rosa Ana
AB Background: Neural stem and progenitor cells (NSPCs) from extra-neural origin represent a valuable tool for autologous cell therapy and research in neurogenesis. Identification of proneurogenic biomolecules on NSPCs would improve the success of cell therapies for neurodegenerative diseases. Preliminary data suggested that follicle-stimulating hormone (FSH) might act in this fashion. This study was aimed to elucidate whether FSH promotes development, self-renewal, and is proneurogenic on neurospheres (NS) derived from sheep ovarian cortical cells (OCCs). Two culture strategies were carried out: (a) long-term, 21-days NS culture (control vs. FSH group) with NS morphometric evaluation, gene expression analyses of stemness and lineage markers, and immunolocalization of NSPCs antigens; (b) NS assay to demonstrate FSH actions on self-renewal and differentiation capacity of NS cultured with one of three defined media: M1: positive control with EGF/FGF2; M2: control; and M3: M2 supplemented with FSH.Results: In long-term cultures, FSH increased NS diameters with respect to control group (302.90 ± 25.20 μm vs. 183.20 ± 7.63 on day 9, respectively), upregulated nestin (days 15/21), Sox2 (day 21) and Pax6 (days 15/21) and increased the percentages of cells immunolocalizing these proteins. During NS assays, FSH stimulated NSCPs proliferation, and self-renewal, increasing NS diameters during the two expansion periods and the expression of the neuron precursor transcript DCX during the second one. In the FSH-group there were more frequent cell-bridges among neighbouring NS.Conclusions: FSH is a proneurogenic hormone that promotes OCC-NSPCs self-renewal and NS development. Future studies will be necessary to support the proneurogenic actions of FSH and its potential use in basic and applied research related to cell therapy.
PB Springer
SN 1746-6148
YR 2024
FD 2024-08-20
LK https://hdl.handle.net/20.500.14352/107966
UL https://hdl.handle.net/20.500.14352/107966
LA eng
NO González-Gil, A., Sánchez-Maldonado, B., Rojo, C. et al. Proneurogenic actions of follicle-stimulating hormone on neurospheres derived from ovarian cortical cells in vitro. BMC Vet Res 20, 372 (2024). https://doi.org/10.1186/s12917-024-04203-8
NO Ministerio de Economía y Competitividad (España)
NO Universidad Complutense de Madrid
NO Universidad Complutense de Madrid-Banco Santander
NO Fundação para a Ciência e a Tecnologia
DS Docta Complutense
RD 9 abr 2025