RT Journal Article
T1 Tear cytokine profile of glaucoma patients treated with preservative-free or preserved latanoprost
A1 Martínez De La Casa Fernández-Borrella, José María
A1 Pérez Bartolomé, Francisco
A1 Urcelay, Elena
A1 Santiago, José Luis
A1 Moreno Montañes, Javier
A1 Arriola Villalobos, Pedro
A1 Benítez Del Castillo Sánchez, José Manuel
A1 García Feijoo, Julián
AB Purpose: To determine variations in cytokine levels of glaucoma patients treated either with preservative-free latanoprost or preserved latanoprost, relative to healthy individuals. Methods: Tear samples were collected from 39 healthy subjects, 20 glaucoma patients treated with preserved latanoprost, and 20 patients treated with preservative-free latanoprost. A set of 27 inflammatory cytokines was analyzed in each group, including interleukin (IL)-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), interferon (IFN)-γ, interferon gamma-induced protein (IP)-10, monocyte chemo attractant protein (MCP)-1MCAF, macrophage inflammatory protein (MIP)-1α, MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF). Cytokine concentrations were obtained by the Bio-Plex Human Cytokine Immunoassay. Non-invasive tear breakup time (NI-TBUT), tear meniscus height, corneal fluorescein staining, conjunctival hyperemia and ocular surface disease index (OSDI) were assessed in patients treated with preservative-free and preserved latanoprost. Results: The levels of IL-2, IL-5, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, FGF basic, PDGF-BB, and TNF-α were significantly higher in patients receiving preserved latanoprost, compared to normal controls (p < 0.05). The expression of all the cytokines studied remained statistically invariable in patients receiving preservative-free latanoprost, compared to healthy subjects (p > 0.05). Ocular surface parameters were not significantly different in both glaucoma groups, and no correlation between these clinical parameters and cytokine levels was observed. Conclusions: Treatment with preserved latanoprost has a direct impact on tear cytokine levels, whereas this effect is not observed upon preservative-free latanoprost instillation.
PB Elsevier
SN 1542-0124
YR 2017
FD 2017-03
LK https://hdl.handle.net/20.500.14352/17803
UL https://hdl.handle.net/20.500.14352/17803
LA eng
NO Martínez De La Casa Fernández-Borrella, J. M., Pérez Bartolomé, F:, Urcelay, E. et al. «Tear Cytokine Profile of Glaucoma Patients Treated with Preservative-Free or Preserved Latanoprost». The Ocular Surface, vol. 15, n.o 4, octubre de 2017, pp. 723-29. DOI.org (Crossref), https://doi.org/10.1016/j.jtos.2017.03.004.
NO Redes Temáticas de Investigación Cooperativa en Salud (España)
NO Universidad Complutense de Madrid
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 19 mar 2026