RT Journal Article
T1 The Diaphanous-related Formin FHOD1 Associates with ROCK1 and Promotes Src-dependent Plasma Membrane Blebbing
A1 Hannemann, Sebastian
A1 Madrid González, Ricardo
A1 Stastna, Jana
A1 Kitzing, Thomas
A1 Gasteier, Judith
A1 Schönichen, André
A1 Bouchet, Jerome
A1 Jimenez García, Alberto
A1 Geyer, Matthias
A1 Grosse, Robert
A1 Benichou, Serge
A1 Fackler, Oliver
AB Diaphanous-related formins (DRFs) mediate GTPase-triggered actin rearrangements to regulate central cellular processes, such as cell motility and cytokinesis. The DRF FHOD1 interacts with the Rho-GTPase Rac1 and mediates formation of actin stress fibers in its deregulated form; the physiologically relevant activities and molecular mechanisms of endogenous FHOD1, however, are still unknown. Here we report that FHOD1 physically associates via the N-terminal part of its FH2 domain with the central domain of ROCK1. Although FHOD1 does not affect the kinase activity of ROCK1, the DRF is an efficient substrate for phosphorylation by ROCK1. Co-expression of FHOD1 and ROCK1 results in the generation of nonapoptotic plasma membrane (PM) blebs, to which the DRF is efficiently recruited. Blebbing induced by FHOD1 and ROCK1 depends on F-actin integrity, the Rho-ROCK cascade, and Src activity and is reminiscent of the recently described PM blebs triggered by expression of Src homology 4 (SH4) domain PM targeting signals. Consistently, endogenous FHOD1 is required in SH4 domain expressing cells for efficient PM blebbing and rounded cell morphology in two-dimensional cultures or three-dimensional matrices, respectively. Efficient association of FHOD1 with ROCK1, as well as recruitment of the DRF to blebs, depends on Src activity, suggesting that the functional interaction between both proteins is regulated by Src. These results define a role for endogenous FHOD1 in SH4 domain-induced blebbing and suggest that its activity is regulated by ROCK1 in a Src-dependent manner.
PB Elsevier
SN 0021-9258
YR 2008
FD 2008
LK https://hdl.handle.net/20.500.14352/97593
UL https://hdl.handle.net/20.500.14352/97593
LA eng
NO Hannemann, Sebastian, et al. «The Diaphanous-Related Formin FHOD1 Associates with ROCK1 and Promotes Src-Dependent Plasma Membrane Blebbing». Journal of Biological Chemistry, vol. 283, n.o 41, octubre de 2008, pp. 27891-903. https://doi.org/10.1074/jbc.M801800200.
DS Docta Complutense
RD 16 abr 2025