RT Journal Article
T1 The nitric oxide donor SNAP-induced amino acid neurotransmitter release in cortical neurons. Effects of blockers of voltage-dependent sodium and calcium channels
A1 Merino Martín, José Joaquín
A1 Arce De Obieta, Carmen
A1 Bellver-Landete, Victor
A1 Naddaf, Ahmad
A1 Oset Gasque, María Jesús
A1 González Prieto, María Del Pilar
AB AbstractBackground: The discovery that nitric oxide (NO) functions as a signalling molecule in the nervous system has radically changed the concept of neuronal communication. NO induces the release of amino acid neurotransmitters but the underlying mechanisms remain to be elucidated.Findings: The aim of this work was to study the effect of NO on amino acid neurotransmitter release (Asp, Glu, Gly and GABA) in cortical neurons as well as the mechanism underlying the release of these neurotransmitters. Cortical neurons were stimulated with SNAP, a NO donor, and the release of different amino acid neurotransmitters was measured by HPLC. The involvement of voltage dependent Na+ and Ca2+ channels as well as cGMP in its mechanism of action was evaluated.Conclusions: Our results indicate that NO induces release of aspartate, glutamate, glycine and GABA in cortical neurons and that this release is inhibited by ODQ, an inhibitor of soluble guanylate cyclase. Thus, the NO effect on amino acid neurotransmission could be mediated by cGMP formation in cortical neurons. Our data also demonstrate that the Na+ and Ca2+ voltage- dependent calcium channels are involved in the NO effects on cortical neurons.
PB Plos
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/115953
UL https://hdl.handle.net/20.500.14352/115953
LA eng
NO Merino JJ, Arce C, Naddaf A, Bellver-Landete V, Oset-Gasque MJ, González MP. The nitric oxide donor SNAP-induced amino acid neurotransmitter release in cortical neurons. Effects of blockers of voltage-dependent sodium and calcium channels. PLoS One. 2014 Mar 5;9(3):e90703. doi: 10.1371/journal.pone.0090703. PMID: 24598811; PMCID: PMC3944624.
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 21 abr 2025