RT Journal Article
T1 Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar
A1 Barasona García-Arévalo, José Ángel
A1 Barroso Arévalo, Sandra
A1 Rivera Arroyo, Belén
A1 Gortázar, Christian
A1 Sánchez-Vizcaíno Rodríguez, José Manuel
AB The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC.
PB MDPI
SN 2076-0817
YR 2020
FD 2020-03-25
LK https://hdl.handle.net/20.500.14352/8057
UL https://hdl.handle.net/20.500.14352/8057
LA eng
NO Unión Europea. Horizonte 2020
NO Ministerio de Ciencia e Innovación (MICINN)
NO Ministerio de Economía y Competitividad (MINECO)/Universidad Complutense
DS Docta Complutense
RD 18 abr 2025