RT Journal Article
T1 Physiological effects on rabbit sperm and reproductive response to recombinant rabbit beta nerve growth factor administered by intravaginal route in rabbit does
A1 Sánchez Rodríguez, Ana
A1 Arias Álvarez, María
A1 Millán Pastor, María Pilar
A1 Lorenzo González, Pedro Luis
A1 García García, Rosa María
A1 García Rebollar, Pilar
AB Beta nerve growth factor (β-NGF) is present in the seminal plasma of some species, including rabbits, acting as an ovulation-inducing factor in camelids. Traditionally, GnRH analogues are used to induce ovulation by intramuscular route when artificial insemination (AI) is performed in rabbit does. A specific rabbit recombinant β-NGF (rrβ-NGF) produced in our laboratory was tested as an alternative method to conventional treatment with GnRH analogues to induce ovulation. In the present work, different concentrations (0, 20, 100 ng/mL and 1, 20 and 100 μg/mL) of rrβ-NGF were added to diluted semen to assess its effect on sperm traits (viability and motility parameters). rrβ-NG was used also, incorporated to the AI dose, to evaluate ovulation response (LH and progesterone plasma concentrations, ovulation rate (OR) and embryo implantation at Day 7) after intravaginal administration. A negative control group stimulated with an empty catheter, and a positive control group inseminated and intramuscularly treated as usual with GnRH were also set up. Results showed that seminal quality was influenced by rrβ-NGF depending on the concentration added, being the highest concentrations tested deleterious for semen. Whereas the highest OR was found in the positive control group (100%), concentrations of 20 ng/mL, 1 μg/mL and 20 μg/mL of rrβ-NGF triggered intermediate OR (30, 60 and 42.9%, respectively), and 100 ng/mL and 100 μg/mL had the lowest OR (20 and 14.3%, respectively). Although LH peak was not observed in the first 2 h after AI in the ovulated females from rrβ-NGF groups, plasma progesterone significantly increased at Day 7, except in those females treated with 20 and 100 μg/mL. Also, 98.4% of ovulated females were pregnant on Day 7. In conclusion, rrβ-NGF added to diluted semen affects seminal quality and provokes ovulation, the development of functional CL and conception by intravaginal route in rabbit does, depending on the concentration added.
PB Elsevier
SN 0093-691X
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/93720
UL https://hdl.handle.net/20.500.14352/93720
LA eng
NO Sanchez-Rodriguez, Ana, et al. «Physiological Effects on Rabbit Sperm and Reproductive Response to Recombinant Rabbit Beta Nerve Growth Factor Administered by Intravaginal Route in Rabbit Does». Theriogenology, vol. 157, noviembre de 2020, pp. 327-34. https://doi.org/10.1016/j.theriogenology.2020.08.003.
NO Credit authorship contribution statementAna Sanchez-Rodriguez: Conceptualization, Methodology, Formal analysis, Investigation, Writing - original draft. María Arias-Álvarez: Methodology, Resources, Writing - review & editing, Funding acquisition. Pilar Millán: Methodology, Resources, Writing - review & editing. Pedro L. Lorenzo: Conceptualization, Resources, Writing - review & editing, Supervision, Funding acquisition. Rosa M. García-García: Conceptualization, Methodology, Formal analysis, Investigation, Writing - review & editing, Supervision, Funding acquisition. Pilar G. Rebollar: Conceptualization, Methodology, Formal analysis, Investigation, Resources, Writing - review & editing, Supervision, Funding acquisition.
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 10 abr 2025