RT Journal Article
T1 Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer
A1 Viñes, Joaquim
A1 Cuscó, Anna
A1 Napp, Sebastian
A1 Alvarez, Julio
A1 Sáez Llorente, José Luis
A1 Rosàs Rodoreda, Montserrat
A1 Francino, Olga
A1 Migura García, Lourdes
A1 Álvarez Sánchez, Julio
AB Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1–mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from pigs, and one from the farmer) contained mcr-1 associated with plasmids (IncX4, IncI2, and IncHI2), except for two that yielded mcr-1 in the chromosome. Similar plasmids were distributed in different E. coli lineages. Transmission of mcr-1 to the farmer most likely occurred by horizontal gene transfer from E. coli of calf origin, since plasmids were highly similar (99% coverage, 99.97% identity). Moreover, 33 virulence factors, including stx2 for Shiga toxin E. coli (STEC) were detected, highlighting the role of livestock as a reservoir of pathotypes with zoonotic potential.
PB MPDI
SN 2079-6382
YR 2021
FD 2021-03-17
LK https://hdl.handle.net/20.500.14352/7122
UL https://hdl.handle.net/20.500.14352/7122
LA eng
NO Ministerio de Economía, Industria y Competitividad (MINECO)
NO Generalitat de Cataluña
DS Docta Complutense
RD 19 may 2024