RT Journal Article
T1 Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
A1 Sánchez Calabuig, María Jesús
A1 García Vazquez, Francisco Alberto
A1 Laguna Barraza, Ricardo
A1 Barros García, Carlos
A1 García Párraga, Daniel
A1 Rizos, Dimitrios
A1 Gutiérrez Adán, Alfonso
A1 Pérez Gutiérrez, José Félix
AB The use of cryopreserved dolphin spermatozoa facilitates the exchange of genetic material between aquatic parks and makes spermatozoa accessible to laboratories for studies to further our understanding of marine mammal reproduction. Heterologous IVF, a replacement for homologous IVF, could provide a means to test the sperm fertility potential; to study gamete physiology and early embryo development; and to avoid the use of valuable dolphin oocytes, which are difficult to obtain. Here, we present protocols that have been successfully used to collect and cryopreserve dolphin spermatozoa. The collection of semen is performed by manual stimulation on trained dolphins. Cryopreservation is accomplished using a TRIS egg-yolk based extender with glycerol. In addition, we present a protocol that describes heterologous IVF using dolphin spermatozoa and bovine oocytes and that verifies the hybrid nature of the resulting embryo using PCR. Heterologous fertilization raises questions on fertilization and can be used as a tool to study gamete physiology and early embryo development. In addition, the success of heterologous IVF demonstrates the potential of this technique to test dolphin sperm fertilizing capacity, which is worth further examination.
PB MyJove Corporation
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/117854
UL https://hdl.handle.net/20.500.14352/117854
LA eng
NO Sánchez-Calabuig, M. J., García-Vázquez, F. A., Laguna-Barraza, R., Barros-García, C., García-Parraga, D., Rizos, D., Gutiérrez Adan, A., & Pérez-Gutíerrez, J. F. (2017). Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization. Journal of visualized experiments : JoVE. https://doi.org/10.3791/55237
NO Ministerio de Economía, Comercio y Empresa ( España)
NO Fundación Séneca - Agencia de Ciencia y Tecnología de la Región de Murcia
DS Docta Complutense
RD 10 abr 2025