RT Journal Article T1 Novel biocatalysts for glycerol conversion into 2,3-butanediol A1 Ripoll, Vanessa A1 de Vicente, Gonzalo A1 Morán, Bruno A1 Rojas, Antonia A1 Segarra, Silvia A1 Montesinos, Alejandro A1 Tortajada, Marta A1 Ramón, Daniel A1 Ladero Galán, Miguel A1 Santos Mazorra, Victoria Eugenia AB Bioconversion of biodiesel-derived waste glycerol into high-value products is proposed as a solution to improve economic viability of biorefineries. Several microorganisms are able to metabolize glycerol into 2,3-butanediol (2,3-BD), a promising bulk chemical with wide applications: solvent, fuel additive, and feedstock for synthetic rubber production, among them. In the present work, a wide screening of microorganisms present both into the waste water treatment system in a biodiesel industry and in culture collections was carried out in order to evaluate their potential as new 2,3-BD producer biocatalysts. Two microorganisms for 2,3-BD production from glycerol as sole carbon source have been selected, namely Raoultella planticola CECT 843 and Raoultella terrigena CECT 4519. Raoultella strains belong to the non‐pathogenic bacteria class (biosafety level 1). This genus has not been previously described as biocatalyst for the studied process. The influence of operational temperature, organic acid addition, and yeast extract concentration on 2,3-BD yield and productivity have been studied through Taguchi design methodology as well as initial glycerol concentration. Based on these results, the feasibility of the process employing pure glycerol and different samples of raw glycerol has been demonstrated. PB Elsevier SN 1359-5113 YR 2016 FD 2016 LK https://hdl.handle.net/20.500.14352/100246 UL https://hdl.handle.net/20.500.14352/100246 LA eng NO Ripoll, V., de Vicente, G., Morán, B., Rojas, A., Segarra, S., Montesinos, A., ... & Santos, V. E. (2016). Novel biocatalysts for glycerol conversion into 2, 3-butanediol. Process Biochemistry, 51(6), 740-748. NO This work has been supported under contract EUI2008-03600 and CTQ2010-11765-E. NO Ministerio de Economía, Industria y Competitividad (España) DS Docta Complutense RD 19 abr 2025