%0 Journal Article
%A Gómez-Massa, Elena
%A Lasa-Lázaro, María
%A Gil-Etayo, Francisco Javier
%A Ulloa-Márquez, Esperanza
%A Justo, Iago
%A Loinaz Segurola, Carmelo
%A Calvo Pulido, Jorge
%A Paz Artal, Estela Natividad
%A Talayero, Paloma
%T Donor helper innate lymphoid cells are replaced earlier than lineage positive cells and persist long-term in human intestinal grafts – a descriptive study
%D 2020
%U https://hdl.handle.net/20.500.14352/131900
%X Intestinal grafts carry large donor lymphoid load that is replaced by recipient cells. The dynamics of this process may influence the tolerance, rejection or graft-versus-host disease. We analysed distribution and turnover of T and B (Lin+) lymphocytes, natural killer (NK) and helper innate lymphoid cells (hILC) in intestinal epithelium (IEp) and lamina propia (LP) from a long-term cohort of eight intestinal recipients and from a single patient monitored deeply during the first 8 months post-transplant (posTx). Long-term intestinal grafts showed significantly higher %hILC than native bowels in IEp and LP until 10 years posTx and recovery to normal levels was observed afterwards. We also observed an imbalance between hILC subsets in IEp [increase of type 1 (ILC1) and decrease in type 3 (ILC3) innate lymphoid cells] that persisted along posTx time even when %hILC was similar to native bowels. Regarding hILC origin, we still detected the presence of donor cells at 13 years posTx. However, this chimerism was significantly lower than in Lin+ and NK populations. According to these findings, observation from the patient monitored in early posTx period showed that recipient hILC repopulate earlier and faster than Lin+ cells, with increase in ILC1 related to rejection and infection episodes.
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