RT Book, Section T1 Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques A1 Cases, Bárbara A1 Pastor Vargas, Carlos A1 Perez Gordo, Marina AB The development of techniques and methods for allergen purification is essential for diagnosis and the development of safe immunotherapeutic agents. The most common purification techniques include chromatographic methodologies. In this chapter, we review and describe the details of the methodologies of using ion-exchange, gel-filtration, and affinity chromatography to purify two well-known panallergens, profilin and parvalbumin. PB Springer SN 9781493969234 SN 9781493969258 SN 1064-3745 SN 1940-6029 YR 2017 FD 2017 LK https://hdl.handle.net/20.500.14352/109060 UL https://hdl.handle.net/20.500.14352/109060 LA eng NO Cases B, Pastor-Vargas C, Perez-Gordo M. Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques. Methods Mol Biol. 2017;1592:13-22. doi: 10.1007/978-1-4939-6925-8_2. PMID: 28315208. NO Instituto de Salud Carlos III NO CEU San Pablo Universidad de Madrid DS Docta Complutense RD 5 abr 2025