RT Conference Proceedings
T1 Nutritional iron deficiency effects on microbiota-gut-brain axis
A1 Quarta, Elisa
A1 Bourqqia Ramzi, Marwane
A1 Muñoz Rodríguez, David
A1 García Esteban, María Teresa
A1 Murciano Cespedosa, Antonio
A1 Mateos González, Álvaro
A1 Mansilla Guardiola, Jesús
A1 Lombardo Hernandez, Juan
A1 Baroni, Simona
A1 Geninatti Crich, Simonetta
A1 Geuna, Stefano
A1 Munaron, Luca Maria
A1 Chiabrando, Deborah
A1 Herrera Rincón, Celia
AB Iron deficiency (ID) is the most frequent nutritional deficiency in the world. While it is clear that nutritional ID has an impact on the metabolism and cellular biochemistry of gut bacteria, the potential implications in the microbiota-gut-brain axis (MGB) are poorly understood. Indeed, dysbiosis in the gut microbiota, observed in early-life ID, is associated with neurodevelopmental impairments, including autism spectrum disorder and attention deficit hyperactivity disorder. Understanding the effects of ID on bacterial signaling offers avenues for targeted interventions to mitigate neurodevelopmental risks in iron deficient populations. In this study, we investigated the responses of two representative bacterial species of human microbiota, Escherichia coli (E. coli) and Limosilactobacillus reuteri (L. reuteri), to experimental conditions of ID. Using the iron chelator 2,2’-Bipyridyl (BP), we developed a reliable method for the creation of in-vitro ID conditions on bacteria cells. Then, we assessed and mathematically modelled their growth and cultivability, and we explored the bioelectric profile (electroma) of these bacteria as a potential way of communication with host neurons, using the voltage-sensitive fluorescent dye DiBAC4(3). Our findings revealed that E. coli and L. reuteri behave differentially in response to ID in their culture environment: whereas L. reuteri showed no alterations in growth dynamics neither cultivability when treated with BP, E. coli showed clear decreases in the growth rate and cultivability when affected by ID. Interestingly, only E. coli demonstrated an altered bioelectric profile under conditions of ID, characterized by increased depolarization of cells. Our observations underscore the heterogeneity of bacterial responses to iron deficiency and highlight the complexity of interactions within the gut microbiota. Understanding such variability is crucial for deciphering the role of microbiota in health and disease, particularly in conditions associated with nutritional iron imbalance and neurological disorders.
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/130748
UL https://hdl.handle.net/20.500.14352/130748
LA eng
DS Docta Complutense
RD 25 ene 2026