RT Journal Article
T1 Deletion of the SKO1 Gene in a hog1 Mutant Reverts Virulence in Candida albicans
A1 Urrialde, Verónica
A1 Prieto Prieto, Antonio Daniel
A1 Hidalgo Vico, Susana
A1 Román González, Elvira
A1 Pla Alonso, Jesús
A1 Alonso Monge, Rebeca María Del Mar
AB Candida albicans displays the ability to adapt to a wide variety of environmental conditions, triggering signaling pathways and transcriptional regulation. Sko1 is a transcription factor that was previously involved in early hypoxic response, cell wall remodeling, and stress response. In the present work, the role of sko1 mutant in in vivo and ex vivo studies was explored. The sko1 mutant behaved as its parental wild type strain regarding the ability to colonize murine intestinal tract, ex vivo adhesion to murine gut epithelium, or systemic virulence. These observations suggest that Sko1 is expendable during commensalism or pathogenesis. Nevertheless, the study of the hog1 sko1 double mutant showed unexpected phenotypes. Previous researches reported that the deletion of the HOG1 gene led to avirulent C. albicans mutant cell, which was, therefore, unable to establish as a commensal in a gastrointestinal murine model. Here, we show that the deletion of sko1 in a hog1 background reverted the virulence of the hog1 mutant in a systemic infection model in Galleria mellonella larvae and slightly improved the ability to colonize the murine gut in a commensalism animal model compared to the hog1 mutant. These results indicate that Sko1 acts as a repressor of virulence related genes, concluding that Sko1 plays a relevant role during commensalism and systemic infection.
PB MDPI
SN 2309-608X
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/12676
UL https://hdl.handle.net/20.500.14352/12676
LA eng
NO Urrialde, V., Prieto Prieto, A. D., Hidalgo Vico, S. et al. «Deletion of the SKO1 Gene in a Hog1 Mutant Reverts Virulence in Candida Albicans». Journal of Fungi, vol. 5, n.o 4, noviembre de 2019, p. 107. DOI.org (Crossref), https://doi.org/10.3390/jof5040107.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 14 dic 2025