RT Journal Article
T1 Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep
A1 Pérez de Diego, Ana Cristina
A1 Sánchez Cordón, Pedro José
A1 Heras, Ana Isabel de las
A1 Sánchez-Vizcaíno Rodríguez, José Manuel
AB The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes.
PB Hindawi Publishing Corporation
SN 1537-744X
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/45198
UL https://hdl.handle.net/20.500.14352/45198
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Unión Europea. FP6
DS Docta Complutense
RD 6 may 2024