RT Journal Article
T1 Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis
A1 Dieli, Francesco
A1 Garrido, Joseba M.
A1 Sevilla, Iker A.
A1 Beltrán-Beck, Beatriz
A1 Minguijón, Esmeralda
A1 Ballesteros, Cristina
A1 Galindo, Ruth C.
A1 Boadella, Mariana
A1 Lyashchenko, Konstantin P.
A1 Romero Martínez, Beatriz
A1 Geijo, Maria Victoria
A1 Ruiz Fons, Francisco
A1 Aranaz Martín, Alicia
A1 Juste, Ramón A.
A1 Vicente, Joaquín
A1 de la Fuente, José
A1 Gortázar, Christian
AB Tuberculosis (TB) caused by Mycobacterium bovis and closely related members of the Mycobacterium tuberculosis complex continues to affect humans and animals worldwide and its control requires vaccination of wildlife reservoir species such as Eurasian wild boar (Sus scrofa). Vaccination efforts for TB control in wildlife have been based primarily on oral live BCG formulations. However, this is the first report of the use of oral inactivated vaccines for controlling TB in wildlife. In this study, four groups of 5 wild boar each were vaccinated with inactivated M. bovis by the oral and intramuscular routes, vaccinated with oral BCG or left unvaccinated as controls. All groups were later challenged with a field strain of M. bovis. The results of the IFN-gamma response, serum antibody levels, M. bovis culture, TB lesion scores, and the expression of C3 and MUT genes were compared between these four groups. The results suggested that vaccination with heat-inactivated M. bovis or BCG protect wild boar from TB. These results also encouraged testing combinations of BCG and inactivated M. bovis to vaccinate wild boar against TB. Vaccine formulations using heat-inactivated M. bovis for TB control in wildlife would have the advantage of being environmentally safe and more stable under field conditions when compared to live BCG vaccines. The antibody response and MUT expression levels can help differentiating between vaccinated and infected wild boar and as correlates of protective response in vaccinated animals. These results suggest that vaccine studies in free-living wild boar are now possible to reveal the full potential of protecting against TB using oral M. bovis inactivated and BCG vaccines
SN 1932-6203
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/45199
UL https://hdl.handle.net/20.500.14352/45199
LA eng
NO Unión Europea. FP7
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
DS Docta Complutense
RD 13 oct 2024