RT Journal Article
T1 Sulphur and biothiol metabolism determine toxicity responses and fate of mercury in Arabidopsis
A1 Sobrino Plata, Juan
A1 Barón-Sola, Ángel
A1 Ortega-Villasante, Cristina
A1 Ortega-Campayo, Víctor
A1 González-Berrocal, César
A1 Conesa-Quintana, Carlos
A1 Carrasco-Gil, Sandra
A1 Muñoz-Pinilla, María
A1 Abadía, Javier
A1 Álvarez-Fernández, Ana
A1 Hernández, Luis
AB Mercury (Hg) is one of the most hazardous pollutants released by humans and is of global environmental concern. Mercury causes oxidative stress and strong cellular damages in plants, which can be attenuated by the biosynthesis of thiol-rich peptides (biothiols), including glutathione (GSH) and phytochelatins (PCs). We analysed Hg tolerance and speciation in five Arabidopsis thaliana genotypes, the wild-type Col-0, three knockdown γ-glutamylcysteine synthetase (γECS) mutants and a knockout PC synthase (PCS) mutant. Mercury-PC complexes were detected in roots by HPLC-ESI-TOFMS, with its abundance being limited in γECS mutants. Analysis of Hg-biothiol complexes in the xylem sap revealed that HgPC2 occurs in wild-type Col-0 Arabidopsis, suggesting that Hg could be translocated associated with thiol-rich metabolites. Twenty genes involved in sulphur assimilation, GSH and PCs synthesis were differentially expressed in roots and shoots, implying a complex regulation, possibly involving post-translational mechanisms independent of GSH cellular levels. In summary, the present study describes the importance of biothiol metabolism and adequate GSH levels in Hg tolerance and identifies for the first time Hg-PC complexes in the xylem sap. This finding supports the notion that Hg-biothiol complexes could contribute to Hg mobilisation within plants.
PB Elsevier
SN 0098-8472
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/99598
UL https://hdl.handle.net/20.500.14352/99598
LA eng
NO Sobrino-Plata, Juan, et al. «Sulphur and Biothiol Metabolism Determine Toxicity Responses and Fate of Mercury in Arabidopsis». Environmental and Experimental Botany, vol. 182, febrero de 2021, p. 104302.  https://doi.org/10.1016/j.envexpbot.2020.104302.
NO AcknowledgmentsWork supported by the Spanish State Research Agency (AEI) co-financed with the European Regional Development Fund (ERDF) (projects AGL2014-53771-R and AGL2017-87591-R to LEH and AGL2016-75226-R to AAF and JA; AEI/ERDF, UE). We thank Dr. M. Isabel Orús (Dept. Biology UAM, Madrid, Spain) for help in using the Scholander pressure chamber for obtaining xylem samples.
NO Ministerio de Ciencia e Innovación (España)
NO European Commission
DS Docta Complutense
RD 9 abr 2025