RT Journal Article
T1 Useful Oriented Immobilization of Antibodies on Chimeric Magnetic Particles: Direct Correlation of Biomacromolecule Orientation with Biological Activity by AFM Studies
A1 Marciello, Marzia
A1 Filice, Marco
A1 Olea, David
A1 Velez, Marisela
A1 Guisan, José M.
A1 Mateo, César
AB The preparation and performance of a suitable chimeric biosensor basedon antibodies (Abs) immobilized on lipase-coated magnetic particles by means of astanding orienting strategy are presented. This novel system is based on hydrophobicmagnetic particles coated with modified lipase molecules able to orient and furtherimmobilize different Abs in a covalent way without any previous site-selective chemicalmodification of biomacromolecules. Different key parameters attending the process werestudied and optimized. The optimal preparation was performed using a controlled loading(1 nmol Ab g−1chimeric support) at pH 9 and a short reaction time to recover abiological activity of about 80%. AFM microscopy was used to study and confirm the Abs-oriented immobilization on lipase-coated magnetic particles and thefinal achievement of ahighly active and recyclable chimeric immune sensor. This direct technique wasdemonstrated to be a powerful alternative to the indirect immunoactivity assay methodsfor the study of biomacromolecule-oriented immobilizations.
SN 0743-7463
YR 2014
FD 2014-12-04
LK https://hdl.handle.net/20.500.14352/100808
UL https://hdl.handle.net/20.500.14352/100808
LA eng
NO Marciello M, Filice M, Olea D, Velez M, Guisan JM, Mateo C. Useful Oriented Immobilization of Antibodies on Chimeric Magnetic Particles: Direct Correlation of Biomacromolecule Orientation with Biological Activity by AFM Studies. Langmuir 2014;30:15022–30. https://doi.org/10.1021/la502972v.
NO Centro Superior de Investigaciones Científicas
NO European Commission
DS Docta Complutense
RD 17 abr 2025