RT Journal Article
T1 Stimulation of stem cell niches and tissue regeneration in mouse skin by switchable protoporphyrin IX-dependent photogeneration of reactive oxygen species in situ
A1 Montoya Miñano, Juan José
A1 Espada, Jesús
A1 Carrasco, Elisa
A1 Calvo-Sánchez, María I.
A1 Fernández-Martos, Sandra
AB Here, we describe a protocol to induce switchable in vivo photogeneration of endogenous reactive oxygen species (ROS) in mouse skin. This transient production of ROS in situ efficiently activates cell proliferation in stem cell niches and stimulates tissue regeneration as strongly manifested through the acceleration of burn healing and hair follicle growth processes. The protocol is based on a regulatable photodynamic treatment that treats the tissue with precursors of the endogenous photosensitizer protoporphyrin IX and further irradiates the tissue with red light under tightly controlled physicochemical parameters. Overall, this protocol constitutes an interesting experimental tool to analyze ROS biology.
PB JoVE
YR 2020
FD 2020-05-08
LK https://hdl.handle.net/20.500.14352/115011
UL https://hdl.handle.net/20.500.14352/115011
LA eng
NO Espada, J., Carrasco, E., Calvo-Sánchez, M. I., Fernández-Martos, S., Montoya, J. J. Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ. J. Vis. Exp. (159), e60859, doi:10.3791/60859 (2020).
NO Ministerio de Economía y Competitividad
NO Instituto de Salud Carlos III
NO Comunidad de Madrid
NO Universidad Autónoma de Madrid
DS Docta Complutense
RD 10 abr 2025