RT Journal Article
T1 Paving the Way for Reliable Alzheimer’s Disease BloodDiagnosis by Quadruple Electrochemical Immunosensing
A1 Valverde de la Fuente, Alejandro
A1 Gordón Pidal, José María
A1 Montero Calle, Ana
A1 Arévalo Pérez, Beatriz
A1 Serafín González-Carrato, Verónica
A1 Calero, Miguel
A1 Moreno Guzmán, María
A1 López, Miguel Ángel
A1 Escarpa, Alberto
A1 Yáñez Sedeño, Paloma
A1 Barderas, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values.
PB Wiley
SN 2196-0216
YR 2022
FD 2022-02-01
LK https://hdl.handle.net/20.500.14352/71373
UL https://hdl.handle.net/20.500.14352/71373
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto de Salud Carlos III (ISCIII)
NO Comunidad de Madrid
NO I
DS Docta Complutense
RD 20 ago 2024