RT Journal Article
T1 Serum IgA contributes to the comprehension of Anisakis simplex associated chronic urticaria
A1 González Fernández, Juan
A1 Ullate, Laura
A1 Fernández-Figares Zuleta, Virginia
A1 Rodero Martínez, Marta
A1 Daschner, Alvaro
A1 Cuéllar Del Hoyo, María Del Carmen
AB The phenotype of allergic diseases associated with Anisakis determines the pattern of cytokines related to antibody production. However, the role of serum IgA and the immunomodulatory mechanisms exerted by active infection of L3 or passive mucosal contact with A. simplex specific antigens has not been studied before. We measured serum cytokine by flow cytometry (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, IL-17A, TGF-β1) and antibody levels (IgE, IgG4, IgA) by ELISA against total and excretory-secretory (ES) antigens, Ani s 3,and the group of major allergens Ani s 1, Ani s 7, and Ani s 13 in sera from 10 patients with gastro-allergic anisakiasis (GAA), 11 Anisakis sensitization associated chronic urticaria (CU+) as well as 17 non-Anisakis-sensitized patients with chronic urticaria (CU-), compared with the urticaria control group (18 subjects). Specific IgE, IgG4 and IgA were high in the GAA, but IgA levels were significantly higher in the CU+ with respect the CONTROL group. We observed higher levels of the ratio IgA/IgG4 in CU+ than GAA group for Ani s 1, Ani s 7, Ani s 13 and ES. Furthermore, chronic urticaria (CU) patients showed significant lower levels of IL-10, IFN-γ and IL-17A than patients without CU. The anti-Ani s 13 IgA/IgG4 ratio correlated positively with pro-inflammatory cytokines and ratios (TNF-α, IL-17A, Th17/Th2, Type1/Type2 and TNF-α/IL-10) in CONTROL group. In general, Anti-Anisakis IgA/G4 ratio was high in CU patients. In conclusion, this study demonstrates the importance of serum IgA because it is associated with chronic urticaria independently of Anisakis sensitization.
PB Elsevier
SN 1567-5769
YR 2024
FD 2024-02-07
LK https://hdl.handle.net/20.500.14352/101312
UL https://hdl.handle.net/20.500.14352/101312
LA eng
NO González-Fernández J, Ullate L, Fernández-Fígares V, Rodero M, Daschner A, Cuéllar C. Serum IgA contributes to the comprehension of Anisakis simplex associated chronic urticaria. Int Immunopharmacol. 2024 Feb 7;129:111602. doi: 10.1016/j.intimp.2024.111602. PMID: 38330800.
NO Sociedad Española de Alergología e Inmunología Clínica
NO Fundación Mutua Madrileña
DS Docta Complutense
RD 12 oct 2024