RT Journal Article
T1 Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway.
A1 Sanz Santamaría, Ana Belén
A1 García Sánchez, Raúl
A1 Rodríguez Peña, José Manuel
A1 Díez Muñiz, Sonia
A1 Nombela Cano, César
A1 Peterson, Craig L.
A1 Arroyo Nombela, Francisco Javier
AB In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.
SN 1939-4586
YR 2012
FD 2012-05-23
LK https://hdl.handle.net/20.500.14352/43872
UL https://hdl.handle.net/20.500.14352/43872
LA eng
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Complutense de Madrid
NO European Science Foundation
DS Docta Complutense
RD 8 abr 2025