RT Journal Article
T1 Microglial Activation in the Retina of a Triple-Transgenic Alzheimer’s Disease Mouse Model (3xTg-AD)
A1 García Martín, Elena Salobrar
A1 Rodríguez Neves, Ana C.
A1 Ramírez Sebastián, Ana Isabel
A1 Hoz Montañana, María Rosa de
A1 Fernández Albarral, José Antonio
A1 López Cuenca, Inés
A1 Ramirez Sebastian, Jose Manuel
A1 Ambrósio, Antonio Francisco
A1 Salazar Corral, Juan José
AB Alzheimer’s disease (AD) is the most common type of dementia in the world. The main biomarkers associated with AD are protein amyloid-β (Aβ) plaques and protein tau neurofibrillary tangles, which are responsible for brain neuroinflammation mediated by microglial cells. Increasing evidence has shown that the retina can also be affected in AD, presenting some molecular and cellular changes in the brain, such as microglia activation. However, there are only a few studies assessing such changes in the retinal microglia in animal models of AD. These studies use retinal sections, which have some limitations. In this study, we performed, for the first time in a triple-transgenic AD mouse model (3xTg-AD), a quantitative morphometric analysis of microglia activation (using the anti-Iba-1 antibody) in retinal whole-mounts, allowing visualization of the entire microglial cell, as well as its localization along the extension of the retina in different layers. Compared to age-matched animals, the retina of 3xTg-AD mice presents a higher number of microglial cells and a thicker microglial cell body area. Moreover, the microglia migrate, reorient, and retract their processes, changing their localization from a parallel to a perpendicular position relative to the retinal surface. These findings demonstrate clear microglia remodeling in the retina of 3xTg-AD mice.
PB MDPI
SN 1422-0067
YR 2020
FD 2020-01-27
LK https://hdl.handle.net/20.500.14352/6044
UL https://hdl.handle.net/20.500.14352/6044
LA eng
NO This article belongs to the Special Issue Molecular Mechanism of Alzheimer's Disease IIReceived: 19 December 2019 / Revised: 24 January 2020 / Accepted: 25 January 2020 / Published: 27 January 2020
NO Ministerio de Economía y Competitividad de España (MINECO)/Instituto de Salud Carlos III
NO Instituto de Salud Carlos III/FEDER
NO Santa Casa Mantero Belard Award 2015 (Portugal)
NO Fundação para a. Ciência e a Tecnologia (FCT) (Portugal)
NO Centro 2020 Regional Operational Programme (Portugal)
NO Ministerio de Educación, Cultura y Deporte
NO Ministerio de Ciencia, Innovación y Universidades
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 17 jul 2024