RT Journal Article
T1 Redox Cycling Amplified Electrochemical Lateral-Flow Immunoassay: Toward Decentralized Sensitive Insulin Detection
A1 Nandhakumar, Ponnusamy
A1 Muñoz San Martín, Cristina
A1 Arévalo Pérez, Beatriz
A1 Ding, Shichao
A1 Lunker, Mahika
A1 Vargas, Eva
A1 Djassemi, Omeed
A1 Campuzano Ruiz, Susana
A1 Wang, Joseph
AB While paper-based lateral-flow immunoassays (LFA) offer considerable promise for centralized diagnostic applications, the analytical capability of conventional LFA remains constrained due to the low sensitivity of its common optical detection strategy. To address these issues, we report a simple electrochemical LFA (eLFA) with nanocatalytic redox cycling for decentralized insulin detection. Simultaneous binding of insulin with detection antibodies and capture antibodies through the capillary flow at the LFA platform and signal amplification through the rapid nanocatalytic reduction of [Fe(CN)6]3– (Fe3+) with Au nanoparticles (AuNP) and ammonia-borane (AB), coupled to electrochemical redox cycling reactions involving Fe3+, AuNP, and AB on the carbon working electrode, offer higher sensitivity than conventional colorimetric LFA and enzymatic redox cycling. The resulting integrated eLFA strip allows the detection of low insulin concentrations (LOD = 12 pM) and offers considerable promise for highly sensitive decentralized assays of different biological fluids (saliva and serum) without additional pretreatment or washing steps.
PB ACS Publications
YR 2023
FD 2023-09-21
LK https://hdl.handle.net/20.500.14352/132180
UL https://hdl.handle.net/20.500.14352/132180
LA eng
NO Nandhakumar, Ponnusamy, et al. «Redox Cycling Amplified Electrochemical Lateral-Flow Immunoassay: Toward Decentralized Sensitive Insulin Detection». ACS Sensors, vol. 8, n.o 10, octubre de 2023, pp. 3892-901. DOI.org (Crossref), https://doi.org/10.1021/acssensors.3c01445.
NO Universidad Complutense de Madrid
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 1 abr 2026