%0 Journal Article
%A Valle Noguera, Ana
%A Sancho Temiño, Lucía
%A Castillo González, Raquel
%A Villa Gómez, Cristina
%A Gomez Sánchez, María José
%A Ochoa Ramos, Anne
%A González Granado, José María
%A Cruz Adalia, Aranzazu
%T IL-18-induced HIF-1α in ILC3s ameliorates the inflammation of C. rodentium-induced colitis
%D 2023
%@ 2211-1247
%U https://hdl.handle.net/20.500.14352/92186
%X Group 3 innate lymphoid cells (ILC3s) are vital for defending tissue barriers from invading pathogens. Hypoxia influences the production of intestinal ILC3-derived cytokines by activating HIF. Yet, the mechanisms gov- erning HIF-1a in ILC3s and other innate RORgt+ cells during in vivo infections are poorly understood. In our study, transgenic mice with specific Hif-1a gene inactivation in innate RORgt+ cells (RAG1KO HIF- 1a6Rorc) exhibit more severe colitis following Citrobacter rodentium infection, primarily due to the inability to upregulate IL-22. We find that HIF-1a6Rorc mice have impaired IL-22 production in ILC3s, while non- ILC3 innate RORgt+ cells, also capable of producing IL-22, remain unaffected. Furthermore, we show that IL-18, induced by Toll-like receptor 2, selectively triggers IL-22 in ILC3s by transcriptionally upregulating HIF-1a, revealing an oxygen-independent regulatory pathway. Our results highlight that, during late-stage C. rodentium infection, IL-18 induction in the colon promotes IL-22 through HIF-1a in ILC3s, which is crucial for protection against this pathogen.
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