%0 Journal Article
%A Vicario De La Torre, Marta
%A Caballo González, María
%A Vico Ruiz, Eva
%A Morales Fernández, Laura
%A Arriola Villalobos, Pedro
%A Heras Polo, Beatriz De Las
%A Benítez Del Castillo Sánchez, José Manuel
%A Guzmán Navarro, Manuel
%A Millar, Thomas
%A Herrero Vanrell, María Del Rocío
%A Molina Martínez, Irene Teresa
%T Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film
%D 2018
%@ 2073-4360
%U https://hdl.handle.net/20.500.14352/12761
%X Dry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4°C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 +/- 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 +/- 1.7 mOsm, with a surface tension of 36.5 +/- 0.4 mN/m and viscosity of 3.05 +/- 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute.
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