RT Journal Article
T1 Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film
A1 Vicario De La Torre, Marta
A1 Caballo González, María
A1 Vico Ruiz, Eva
A1 Morales Fernández, Laura
A1 Arriola Villalobos, Pedro
A1 Heras Polo, Beatriz De Las
A1 Benítez Del Castillo Sánchez, José Manuel
A1 Guzmán Navarro, Manuel
A1 Millar, Thomas
A1 Herrero Vanrell, María Del Rocío
A1 Molina Martínez, Irene Teresa
AB Dry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4°C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 +/- 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 +/- 1.7 mOsm, with a surface tension of 36.5 +/- 0.4 mN/m and viscosity of 3.05 +/- 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute.
PB MDPI
SN 2073-4360
YR 2018
FD 2018-04-11
LK https://hdl.handle.net/20.500.14352/12761
UL https://hdl.handle.net/20.500.14352/12761
LA eng
NO Vicario-de-la-Torre M, Caballo-González M, Vico E, Morales-Fernández L, Arriola-Villalobos P, De Las Heras B, et al. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers 2018;10:425. https://doi.org/10.3390/polym10040425.
NO Ministerio de Economía y Competitividad (España)
NO Instituto de Salud Carlos III
NO Federación Española de Enfermedades Raras
DS Docta Complutense
RD 6 abr 2025