RT Journal Article
T1 Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells
A1 Haro-González, P.
A1 Ramsay, W.T.
A1 Martínez Maestro, Laura
A1 Del Rosal, B.
A1 Santacruz-Gomez, K.
A1 Iglesias-De La Cruz, M.D.C.
A1 Sanz-Rodríguez, F.
A1 Chooi, J.Y.
A1 Sevilla, P.R.
A1 Bettinelli, M.
A1 Choudhury, D.
A1 Kar, A.K.
A1 Solé, J.G.
A1 Jaque, D.
A1 Paterson, L.
AB Laser-induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 degrees C), thus, avoiding cell damage.
PB Wiley
SN 1613-6810
YR 2013
FD 2013-02-11
LK https://hdl.handle.net/20.500.14352/97577
UL https://hdl.handle.net/20.500.14352/97577
LA eng
NO Haro-González, P., Ramsay, W.T., Maestro, L.M., del Rosal, B., Santacruz-Gomez, K., del Carmen Iglesias-de la Cruz, M., Sanz-Rodríguez, F., Chooi, J.Y., Sevilla, P.R., Bettinelli, M., Choudhury, D., Kar, A.K., Solé, J.G., Jaque, D. and Paterson, L. (2013), Quantum Dot-Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells. Small, 9: 2162-2170. https://doi.org/10.1002/smll.201201740
NO Universidad Autonoma de Madrid
NO Comunidad Autónoma de Madrid
NO Ministerio de Educación y Ciencia (España)
NO Malta Consolider-Ingenio
NO Caja Madrid Foundation
NO Fondazione Cariverona
NO Fundacion Dr. Manuel Morales
NO Banco Santander
NO CEAL-UAM through the UAM-NUS cooperation project
NO UK Research & Innovation (UKRI)
NO Engineering & Physical Sciences Research Council (EPSRC)
NO Heriot Watt University Life Science Interface Theme Scholarship
DS Docta Complutense
RD 18 dic 2025