RT Journal Article
T1 Altered expression of P2Y2 and P2X7 purinergic receptors in the isolated rat heart mediates ischemia-reperfusion injury
A1 Montoya Miñano, Juan José
A1 Granado, Miriam
A1 Amor, Sara
A1 Monge, Luis
A1 Fernández, Nuria
A1 García-Villalón, Angel Luis
AB The aim of this study is to analyze the expression of purinergic receptors in the heart after ischemia-reperfusion, and their possible role in ischemia-reperfusion injury. Rat hearts were perfused according to the Langendorff technique and subjected to 30. min ischemia followed by 15. min reperfusion. Ischemia-reperfusion reduced the gene expression and protein content of purinergic receptors of the P2Y2 subtype, and increased the gene expression and protein content of the P2X7 subtype. Treatment with the agonist of the P2Y2 subtype 2-thio-UTP and with the antagonist of the P2X7 subtype Brilliant Blue improved myocardial function parameters, reduced cell death and increased the myocardial expression of antiapoptotic markers after ischemia-reperfusion. These results suggest that the myocardial expression of the protective P2Y2 subtype of purinergic receptors is reduced, whereas that of the harmful subtype P2X7 subtype is increased during coronary ischemia-reperfusion. This may contribute to myocardial injury in this condition.
PB Science Direct
YR 2015
FD 2015-06-10
LK https://hdl.handle.net/20.500.14352/114915
UL https://hdl.handle.net/20.500.14352/114915
LA eng
NO Granado, M., Amor, S., Montoya, J. J., Monge, L., Fernández, N., & García-Villalón, Á. L. (2015). Altered expression of P2Y2 and P2X7 purinergic receptors in the isolated rat heart mediates ischemia-reperfusion injury. Vascular Pharmacology, 73, 96-103. https://doi.org/10.1016/J.VPH.2015.06.003
NO Fondo de Investigaciones Sanitarias
NO Proyectos UAM-Banco de Santander con EEUU (Convocatoria 2013)
DS Docta Complutense
RD 13 abr 2025