RT Journal Article
T1 Toward Early Diagnosis of Late-Onset Sepsis in Preterm Neonates: Dual Magnetoimmunosensor for Simultaneous Procalcitonin and C-Reactive Protein Determination in Diagnosed Clinical Samples
A1 Molinero-Fernández, Águeda
A1 Moreno Guzmán, María
A1 Arruza Gómez, Luis
A1 López, Miguel Ángel
A1 Escarpa, Alberto
AB Early diagnosis of sepsis, combining blood cultures and inflammation biomarkers, continues to be a challenge, especially in very low birth weight (VLBW) infants because of limited availability of blood samples. Traditional diagnostic procedures are cumbersome, not fast enough, and require relatively large volumes of sample. Empiric use of antibiotics, before diagnostic confirmation, is required to decrease mortality, leading to potential antibiotic resistance and side effects in VLBW infants. To solve such a serious problem, a dual magnetoimmunosensor is proposed for simultaneous assessment of two of the most important sepsis biomarkers: procalcitonin (PCT for early phase) and C-reactive protein (CRP for late phase). This "sample-to-result" approach exhibited excellent sensitivity, selectivity, precision, and stability using low sample volumes (<30 μL) and under 20 min of total assay. The analytical usefulness of the approach was demonstrated by analyzing clinically relevant samples of preterm neonates with suspicion of sepsis.
PB ACS publication
SN 2379-3694
YR 2019
FD 2019-07-15
LK https://hdl.handle.net/20.500.14352/93044
UL https://hdl.handle.net/20.500.14352/93044
LA eng
NO Molinero-Fernández Á, Moreno-Guzmán M, Arruza L, López MÁ, Escarpa A. Toward Early Diagnosis of Late-Onset Sepsis in Preterm Neonates: Dual Magnetoimmunosensor for Simultaneous Procalcitonin and C-Reactive Protein Determination in Diagnosed Clinical Samples. ACS Sens 2019;4:2117–23. https://doi.org/10.1021/acssensors.9b00890.
DS Docta Complutense
RD 20 ago 2024