RT Journal Article
T1 PARAGEN 1.0: A Standardized Synthetic Gene Library for Fast Cell-Free Bacteriocin Synthesis
A1 Gabant, Philippe
A1 Borrero Del Pino, Juan
AB The continuous emergence of microbial resistance to our antibiotic arsenal is widely becoming recognized as an imminent threat to global human health. Bacteriocins are antimicrobial peptides currently under consideration as real alternatives or complements to common antibiotics. These peptides have been much studied, novel bacteriocins are regularly reported and several genomic databases on these peptides are currently updated. Despite this, to our knowledge, a physical collection of bacteriocins that would allow testing and comparing them for different applications does not exist. Rapid advances in synthetic biology in combination with cell-free protein synthesis technologies offer great potential for fast protein production. Based on the amino acid sequences of the mature peptide available in different databases, we have built a bacteriocin gene library, called PARAGEN 1.0, containing all the genetic elements required for in vitro cell-free peptide synthesis. Using PARAGEN 1.0 and a commercial kit for cell-free protein synthesis we have produced 164 different bacteriocins. Of the bacteriocins synthesized, 54% have shown antimicrobial activity against at least one of the indicator strains tested, including Gram-positive and Gram-negative bacteria representing commonly used lab strains, industrially relevant microorganisms, and known pathogens. This bacteriocin collection represents a streamlined pipeline for selection, production, and screening of bacteriocins as well as a reservoir of ready-to-use antimicrobials against virtually any class of relevant bacteria.
PB Frontiers
SN 2296-4185
YR 2019
FD 2019-09-06
LK https://hdl.handle.net/20.500.14352/116430
UL https://hdl.handle.net/20.500.14352/116430
LA eng
NO Gabant P and Borrero J (2019) PARAGEN 1.0: A Standardized Synthetic Gene Library for Fast Cell-Free Bacteriocin Synthesis. Front. Bioeng. Biotechnol. 7:213. doi: 10.3389/fbioe.2019.00213
NO Author Contributions:PG and JB have contributed equally to the design and implementation of the research, to the analysis of the results and to the writing of the manuscript.
DS Docta Complutense
RD 18 abr 2025