RT Journal Article
T1 Bacteria as Nanoparticles Carrier for Enhancing Penetration in a Tumoral Matrix Model
A1 Moreno Zafra, Víctor Manuel
A1 Vallet Regí, María Dulce Nombre
A1 Álvarez Corchado, Elena
A1 Izquierdo Barba, Isabel
A1 Baeza, Alejandro
A1 Serrano López, Juana
AB One of the major concerns in the application of nanocarriers in oncology is their scarce penetration capacity in tumoral tissues, which drastically compromises the effectivity. Living organisms as cells and bacteria present the capacity to navigate autonomously following chemical gradients being able to penetrate deeply into dense tissues. In the recent years, the possibility to employ these organisms for the transportation of therapeutic agents and nanocarriers attached on their membrane or engulfed in their inner space have received huge attention. Herein, based on this principle, a new approach to deliver drug loaded nanoparticles achieving high penetration in tumoral matrices is presented. In this case, Escherichia coli (E. coli) bacteria wall is decorated with azide groups, whereas alkyne-strained groups are incorporated on the surface of mesoporous silica nanoparticles loaded with a potent cytotoxic compound, doxorubicin. Both functional groups form stable triazole bonds by click-type reaction allowing the covalent grafting of nanoparticles on living bacteria. Thus, the motility and penetration capacity of bacteria, which carried nanoparticles are evaluated in a 3D tumoral matrix model composed by a dense collagen extracellular matrix with HT1080 human fibrosarcome cells embedded. The results confirmed that bacteria are able to transport the nanoparticles crossing a thick collagen layer being able to destroy almost 80% of the tumoral cells located underneath. These findings envision a powerful strategy in nanomedicine applied for cancer treatment by Q4 allowing a homogeneous distribution of therapeutic agents in the malignancy.
PB Wiley Online Library
SN 2196-7350
YR 2020
FD 2020-04-21
LK https://hdl.handle.net/20.500.14352/6134
UL https://hdl.handle.net/20.500.14352/6134
LA eng
NO Moreno Zafra, V. M., Álvarez Corchado, E., Izquierdo Barba, I. et al. «Bacteria as Nanoparticles Carrier for Enhancing Penetration in a Tumoral Matrix Model». Advanced Materials Interfaces, vol. 7, n.o 11, junio de 2020, p. 1901942. DOI.org (Crossref), https://doi.org/10.1002/admi.201901942.
NO RESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba)ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba)RESEARCHER ID K-8193-2014 (Alejandro Baeza)ORCID 0000-0002-2026-6266 (Alejandro Baeza)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea
DS Docta Complutense
RD 28 mar 2025