RT Journal Article
T1 Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy
A1 Shimolina, Liubov E.
A1 Gulin, Alexander A.
A1 Paez-Perez, Miguel
A1 López Duarte, Ismael
A1 Druzhkova, Irina N.
A1 Lukina, Maria M.
A1 Gubina, Margarita V.
A1 Brooks, Nicolas J.
A1 Zagaynova, Elena V.
A1 Kuimova, Marina K.
A1 Shirmanova, Marina V.
AB Significance: Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the quantification of membrane viscosity with high spatiotemporal resolution, down to the individual cell organelles.Aim: The aim of our work was to analyze microviscosity of the plasma membrane of living cancer cells during chemotherapy with cisplatin using FLIM and correlate the observed changes with lipid composition and cell's response to treatment.Approach: FLIM together with viscosity-sensitive boron dipyrromethene-based fluorescent molecular rotor was used to map the fluidity of the cell's membrane. Chemical analysis of membrane lipid composition was performed with time-of-flight secondary ion mass spectrometry (ToF-SIMS).Results: We detected a significant steady increase in membrane viscosity in viable cancer cells, both in cell monolayers and tumor spheroids, upon prolonged treatment with cisplatin, as well as in cisplatin-adapted cell line. ToF-SIMS revealed correlative changes in lipid profile of cisplatintreated cells.Conclusions: These results suggest an involvement of membrane viscosity in the cell adaptation to the drug and in the acquisition of drug resistance.
PB SPIE-Intl Soc Optical Eng
SN 1083-3668
YR 2020
FD 2020-12-16
LK https://hdl.handle.net/20.500.14352/115329
UL https://hdl.handle.net/20.500.14352/115329
LA eng
NO Shimolina LE, Gulin AA, Paez-Perez M, López-Duarte I, Druzhkova IN, Lukina MM, et al. Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy. J Biomed Opt [Internet]. 16 de diciembre de 2020 [citado 21 de enero de 2025];25(12). Disponible en: https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-12/126004/Mapping-cisplatin-induced-viscosity-alterations-in-cancer-cells-using-molecular/10.1117/1.JBO.25.12.126004.full
NO Russian Science Foundation
NO Russian Foundation for Basic Research
DS Docta Complutense
RD 10 abr 2025