RT Journal Article
T1 A new hormonal protocol supports early development of in vitro-produced embryos after transfer to anoestrus mares
A1 Muñoz García, Carmen C.
A1 Luis Calero, Marcos
A1 Fernández Hernández, Pablo
A1 Pizarro Hernández, Alba
A1 Fernández González, Raúl
A1 Gutiérrez Adán, Alfonso
A1 Sánchez Calabuig, María Jesús
A1 Cuervo Arango, Juan
A1 González Fernández, Lauro
A1 Macías García, Beatriz
AB The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI-derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling.
PB Wiley
SN 0936-6768
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/109800
UL https://hdl.handle.net/20.500.14352/109800
LA eng
NO Muñoz-García, C. C., Luis-Calero, M., Fernández-Hernández, P., Pizarro-Hernández, A., Fernández-González, R., Gutiérrez-Adán, A., Sánchez-Calabuig, M. J., Cuervo-Arango, J., González-Fernández, L., & Macías-García, B. (2024). A new hormonal protocol supports early development of in vitro-produced embryos after transfer to anoestrus mares. Reproduction in Domestic Animals, 59(Suppl. 3), e14622. https://doi.org/10.1111/rda.14622
NO Marcos Luis-Calero ha contado con el apoyo del ‘Plan Propio de Iniciación a la Investigación, Desarrollo Tecnológico e Innovación. Acción II’ de la Universidad de Extremadura y del Banco Santander; Referencia: RC1. Carmen C. Muñoz-García se financia por el ‘Programa Investigo’ de la Junta de Extremadura; Reference: PI-0152-22.
NO Junta de Extremadura
NO European Commission
DS Docta Complutense
RD 17 abr 2025