RT Journal Article
T1 Antibacterial Effects of Toothpastes Evaluated in an In Vitro Biofilm Model
A1 Fernández, Eva
A1 Sánchez Beltrán, María Del Carmen
A1 Llama Palacios, María Arantxazu
A1 Sanz Alonso, Mariano
A1 Herrera González, David
AB Purpose: To test the antibacterial effects of different toothpastes with the slurry method of toothpaste application in an in vitro oral biofilm model including relevant periodontal pathogens.Materials and methods: Four commercially available toothpastes, two containing sodium fluoride (NaF) at different concentrations (1450 and 2500 ppm), two NaF with either triclosan or stannous fluoride, and a control phosphate-buffered saline (PBS) were used. Multispecies biofilms containing 6 species of oral bacteria were grown on hydroxyapatite disks for 72 h and then exposed for 2 min to the toothpaste slurries or phosphate buffer saline (PBS) by immersion, under continuous agitation at 37°C. Biofilms were then analysed by means of real-time polymerase chain reaction (PCR), combined with propidium monoazide (PMA). Statistical evaluation was performed using ANOVA and Student's t-test, with Bonferroni correction for multiple comparisons.Results: The toothpastes containing NaF and stannous fluoride demonstrated superior antimicrobial activity for A. actinomycetencomitans, P. gingivalis and F. nucleatum when compared to those containing NaF and triclosan, 1450 ppm NaF or 2500 ppm NaF in this multispecies biofilm model.Conclusion: The proposed model for the evaluation of toothpastes in the form of slurries detected significant differences in the antimicrobial effects among the tested NaF-containing toothpastes, with the stannous fluoride-based formulation achieving better results than the other formulations. The use of toothpaste as slurries and real-time PCR with PMA is an adequate method for comparing the in vitro antimicrobial effect of different toothpastes.
PB Quintessence Publishing
SN 1602-1622
YR 2017
FD 2017-07-07
LK https://hdl.handle.net/20.500.14352/126467
UL https://hdl.handle.net/20.500.14352/126467
LA eng
NO Fernández E, Sánchez MDC, Llama-Palacios A, Sanz M, Herrera D. Antibacterial Effects of Toothpastes Evaluated in an 
In Vitro Biofilm Model. Oral Health Prev Dent. 2017;15(3):251-257. doi: 10.3290/j.ohpd.a38526
DS Docta Complutense
RD 19 ene 2026