RT Journal Article
T1 Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites
A1 Matesanz Sancho, María Concepción
A1 Feito Castellano, María José
A1 Oñaderra Sánchez, Mercedes
A1 Ramirez Santillán, Cecilia
A1 Casa, Carmen da
A1 Arcos Navarrete, Daniel
A1 Rojo, José María
A1 Vallet Regí, María Dulce Nombre
A1 Portolés Pérez, María Teresa
AB Hypothesis: Synthetic hydroxyapatite (HA) and Si substituted hydroxyapatite (SiHA) are calcium phosphate ceramics currently used in the field of dentistry and orthopaedic surgery. The preparation of both biomaterials as polycrystalline solid pieces or grains formed by nanocrystallites has awakened a great interest to enhance the bioactive behavior due to the microstructural defects and the higher surface area. The study of the macrophage and lymphocyte behavior in contact with nanocrystalline HA and SiHA will allow to elucidate the immune response which conditions the success or rejection of these biomaterials. Experiments: HA and SiHA granules (with sizes of tens of microns) have been prepared by controlled aqueous precipitation avoiding subsequent high temperature sintering. HA and SiHA granules were constituted by crystallites smaller than 50 nm. The effects of both nanocrystalline materials on immune system have been evaluated with macrophages (main components of innate immune system) and T lymphocytes (specific cells of adaptive response) after short-term culture as in vitro models of the early immune response. Findings: Significant decreases of macrophage proliferation and phagocytic activity, increased production of inflammatory cytokines (IL-6, TNF-a) and T lymphocyte apoptosis, were induced by these nanocrystalline ceramics suggesting that, after in vivo implantation, they induce significant effects on immune responses, including an early activation of the innate immune system. (C) 2013 Elsevier Inc. All rights reserved.
PB Elsevier
SN 0021-9797
YR 2014
FD 2014-02-15
LK https://hdl.handle.net/20.500.14352/35362
UL https://hdl.handle.net/20.500.14352/35362
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovacion (MICINN)
NO Agening Network of Excellence
DS Docta Complutense
RD 9 abr 2025