RT Journal Article
T1 Crosslinks in the cell wall of budding yeast control morphogenesis at the mother-bud neck
A1 Blanco, Noelia
A1 Reidy, Michael
A1 Arroyo, Javier
A1 Cabib, Enrico
AB Previous work has shown that, in cla4Δ cells of budding yeast, where septin ring organization is compromised, the chitin ring at the mother-daughter neck becomes essential for prevention of neck widening and for cytokinesis. Here, we show that it is not the chitin ring per se, but its linkage to β(1-3)glucan that is required for control of neck growth. When in a cla4Δ background, crh1Δ crh2Δ mutants, in which the chitin ring is not connected to β(1-3)glucan, grew very slowly and showed wide and growing necks, elongated buds and swollen cells with large vacuoles. A similar behavior was elicited by inhibition of the Crh proteins. This aberrant morphology matched that of cla4Δ chs3Δ cells, which have no chitin at the neck. Thus, this is a clear case in which a specific chemical bond between two substances, chitin and glucan, is essential for the control of morphogenesis. This defines a new paradigm, in which chemistry regulates growth.
SN 1477-9137
YR 2012
FD 2012-10-17
LK https://hdl.handle.net/20.500.14352/43860
UL https://hdl.handle.net/20.500.14352/43860
LA eng
NO Comunidad de Madrid
NO Ministerio de Educación y Ciencia
NO UCM
DS Docta Complutense
RD 30 abr 2024