RT Journal Article
T1 A chemiluminescence study on degradation of gelatine biodegradation by bacteria and fungi isolated from cinematographic films
A1 Abrusci, Concepción
A1 Marquina Díaz, Domingo
A1 Santos de la Sen, Antonio
A1 Del Amo, Alfonso
A1 Corrales, Teresa
A1 Catalina, Fernando
AB Chemiluminescence (CL) has become a sensitive tool for the study of polymer degradation, induced by exposure to various factors, such us heat, UV-light and oxygen. In this paper, the results obtained with this technique in the study of gelatine samples hydrolytically degraded under sterilisation conditions are presented. Also, photographic gelatine exposed to bacterial and fungal degradations, in water solution and under controlled conditions, have been study by the chemiluminescence emission of their corresponding films and the biodegradation extent was determined by viscosity. The bacteria and fungi employed in this work have been isolated from cinematographic films in a previous work.The high intensities of chemiluminescence emission obtained for gelatines biodegraded by bacteria and fungi, in aqueous solution at 37 and 25 °C, respectively, are different to those obtained in the thermal degradation. The hydrolytic degradation mechanism is through a cleavage of the peptide bond of the protein without significant oxidation of the material. In contrast, biodegradation by bacteria and fungi at low temperatures decreases the molecular weight of the gelatine (viscosity) by the enzymatic activity but, also, produces an important oxidation in the material due to the reactive oxygen species (ROS) generated in the microbial metabolism.
PB Elsevier
SN 1010-6030
YR 2006
FD 2006-07-14
LK https://hdl.handle.net/20.500.14352/106874
UL https://hdl.handle.net/20.500.14352/106874
LA eng
NO Abrusci, C., et al. «A Chemiluminescence Study on Degradation of Gelatine». Journal of Photochemistry and Photobiology A: Chemistry, vol. 185, n.o 2-3, enero de 2007, pp. 188-97. DOI.org (Crossref), https://doi.org/10.1016/j.jphotochem.2006.06.003.
DS Docta Complutense
RD 24 ago 2024