RT Journal Article
T1 Mechanisms involved in the nitric oxide-induced vasorelaxation in porcine prostatic small arteries
A1 Fernandes, Vítor S.
A1 Martínez Sáenz, Ana
A1 Recio Visedo, María Paz
A1 Fernandes Ribeiro, Ana Sofía
A1 Ana Sánchez, 
A1 Martínez Sainz, María Del Pilar
A1 Martínez Gómez, Ana Cristina
A1 García Sacristán, Albino
A1 Orensanz Muñoz, Luis Miguel
A1 Prieto Ocejo, Dolores
A1 Hernández Rodríguez, Medardo Vicente
AB Benign prostatic hypertrophy has been known to be related with glandular ischemia processes, and nitric oxide (NO) is a potent vasodilator agent. Therefore, the current study investigates the mechanisms underlying the NO-induced vasorelaxation in pig prostatic small arteries. In microvascular myographs, relaxation to electrical field stimulation (EFS), or to exogenous (S)-nitroso-N-acetylpenicillamine (SNAP) and acetylcholine (ACh), was observed on noradrenaline-precontracted prostatic small arterial rings under non-adrenergic and non-cholinergic (NANC) conditions. EFS (1-16 Hz) and exogenous SNAP (0.1-30 μM) evoked frequency- and concentration-dependent relaxation, respectively. Tetrodotoxin, a neuronal voltage-gated Na(+) channel blocker, abolished the EFS-evoked relaxation. ACh (1 nM-10 μM) induced concentration-dependent relaxation, which was reduced by the NO synthase inhibitor N(G)-nitro-L: -arginine (L: -NOARG). L: -NOARG also reduced the EFS-elicited relaxation but failed to modify the response to SNAP. 1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and iberiotoxin (IbTX), blockers of soluble guanylyl cyclase and large conductance Ca(2+)-activated K(+) (BK(Ca)) channels, respectively, reduced EFS-, SNAP-, and ACh-induced relaxation. The combination of ODQ with IbTX did not produce further inhibition of the responses to either SNAP or ACh, compared with ODQ alone. Blockade of cyclooxygenases and intermediate and small conductance Ca(2+)-activated, ATP-dependent, and voltage-gated K(+) channels did not change the EFS and SNAP responses. In conclusion, our results suggest that NO and non-NO non-prostanoid factor(s) derived from NANC nerves are involved in the vasodilatation of pig prostatic small arteries. NO produces relaxation through soluble guanylyl cyclase activation-dependent BK(Ca) channel opening and through guanylyl cyclase-independent mechanisms. The vasodilatation elicited by NO could be useful to prevent prostatic ischemia.
PB Springer Link
SN 0028-1298
SN 1432-1912
YR 2011
FD 2011-07-12
LK https://hdl.handle.net/20.500.14352/94315.2
UL https://hdl.handle.net/20.500.14352/94315.2
LA eng
NO Fernandes VS, Martínez-Sáenz A, Recio P, Ribeiro AS, Sánchez A, Martínez MP, Martínez AC, García-Sacristán A, Orensanz LM, Prieto D, Hernández M. Mechanisms involved in the nitric oxide-induced vasorelaxation in porcine prostatic small arteries. Naunyn Schmiedebergs Arch Pharmacol. 2011 Sep;384(3):245-53. doi: 10.1007/s00210-011-0666-2. Epub 2011 Jul 12. PMID: 21748357.
DS Docta Complutense
RD 25 ago 2024