RT Book, Section T1 Brucellosis in aquatic mammals A1 Vargas Castro, Ignacio A1 Hernández-Mora, Gabriela A1 González-Barrientos, Rocío A1 Grattarola, Carla AB The study of brucellosis in marine mammals has become crucial due to its zoonotic risks and its effects on marine species. The bacteria Brucella ceti and Brucella pinnipedialis cause significant health issues in cetaceans and pinnipeds, respectively. While B. ceti is associated with severe conditions and strandings, B. pinnipedialis often remains asymptomatic, though some cases of disease have been noted. The zoonotic potential of marine Brucella strains, particularly sequence type 27, further emphasizes the need for continuous surveillance and research. While real-time PCR is useful for detecting Brucella DNA, it faces limitations, making it unsuitable for direct application to these samples. Bacterial culture remains the gold standard for diagnosing brucellosis, and molecular techniques should be applied to these cultures to ensure accurate strain identification. Additionally, the limitation of standardized serological tests for all marine mammals species underscores the necessity for developing reliable and accesible diagnostic tools, including ELISAs, to improve diagnostic accuracy PB Elsevier SN Brucellosis The Silent Threat to Livestock and Human Health YR 2026 FD 2026 LK https://hdl.handle.net/20.500.14352/129497 UL https://hdl.handle.net/20.500.14352/129497 LA eng NO Vargas-Castro I., Hernandez-Mora G., Gonzalez-Barrientos R. y Grattarola C. (2025) Brucellosis in aquatic mammals. En Brucellosis: The Silent Threat to Livestock and Human Health. Ed. Elsevier 8:133-145. ISBN: 9780443300677. 2025. (Capítulo) DS Docta Complutense RD 25 dic 2025