RT Journal Article
T1 Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
A1 Feito Hermida, Javier
A1 Araújo, Carlos
A1 Arbulu, Sara
A1 Contente, Diogo
A1 Gómez Sala, Beatriz
A1 Díaz-Formoso, Lara
A1 Muñoz Atienza, Estefanía
A1 Borrero Del Pino, Juan
A1 Cintas Izarra, Luis Miguel
A1 Hernández Cruza, Pablo Elpidio
AB Lactococcus garvieae is a main ichthyopathogen in rainbow trout (Oncorhynchus mykiss, Walbaum) farming, although bacteriocinogenic L. garvieae with antimicrobial activity against virulent strains of this species have also been identified. Some of the bacteriocins characterized, such as garvicin A (GarA) and garvicin Q (GarQ), may show potential for the control of the virulent L. garvieae in food, feed and other biotechnological applications. In this study, we report on the design of Lactococcus lactis strains that produce the bacteriocins GarA and/or GarQ, either alone or together with nisin A (NisA) or nisin Z (NisZ). Synthetic genes encoding the signal peptide of the lactococcal protein Usp45 (SPusp45), fused to mature GarA (lgnA) and/or mature GarQ (garQ) and their associated immunity genes (lgnI and garI, respectively), were cloned into the protein expression vectors pMG36c, which contains the P32 constitutive promoter, and pNZ8048c, which contains the inducible PnisA promoter. The transformation of recombinant vectors into lactococcal cells allowed for the production of GarA and/or GarQ by L. lactis subsp. cremoris NZ9000 and their co-production with NisA by Lactococcus lactis subsp. lactis DPC5598 and L. lactis subsp. lactis BB24. The strains L. lactis subsp. cremoris WA2-67 (pJFQI), a producer of GarQ and NisZ, and L. lactis subsp. cremoris WA2-67 (pJFQIAI), a producer of GarA, GarQ and NisZ, demonstrated the highest antimicrobial activity (5.1- to 10.7-fold and 17.3- to 68.2-fold, respectively) against virulent L. garvieae strains.
PB MPDI
SN 2304-8158
YR 2023
FD 2023-03-02
LK https://hdl.handle.net/20.500.14352/72398
UL https://hdl.handle.net/20.500.14352/72398
LA eng
NO Ministerio de Ciencia, Innovación y Universidades
NO Universidad Complutense de Madrid (UCM)
NO Santander/UCM
DS Docta Complutense
RD 27 jul 2024